Fig. 6.

dra1 shows normal levels of PHYA message and phyA protein. (A) Relative PHYA expression in 7-day-old white light (W)-grown seedlings of the wild type (WT) and dra1. PHYA expression was measured by quantitative RT-PCR, normalized to the ACT2 control gene. Data represent the mean ±SE from five independent batches of 20 seedlings. (B) One-day-old etiolated seedlings were either maintained in darkness or transferred to monochromatic red light for 3 d, at which point protein was extracted. Upper panel: western blot of extracts of the WT, dra1, and phyA-410 probed with phyA-specific monoclonal antibodies. Lower panel: Coomassie-stained blot showing equal loading.