Table 2.

Real-time PCR primers

Bacterial strain	Target	Sequence	Product size, bp
Universal for bacteria	16SrRNA	F-ACT ACG TGC CAG CAG CC	296-300
		R-GGA CTA CCA GGG TAT CTA ATC C
S. mutans	gtfB	F-AGC CAT GCG CAA TCA ACA GGT T	415
		R-CGC AAC GCG AAC ATC TTG ATC AG
S. gordonii	arcA	F-GCT ATT CGT GAG TTG CTT CAA GG	107
		R-TTT GCT GCT TCT GGA ATT TCT GG
S. sanguinis	sagP	F-GTG GTG GTG GCA ATA TCG TAG	178
		R-CGA CCT CGA ACC AAT TCA CTT CC
A. naeslundii	ureA	F-ACG AAG ACG CAA GGA CAG AGG	118
		R-GTA GGC CAT GAG ATC CGT GAC C
S. salivarius	ureC	F-AGG TTC AGG TGG TGG ACA TGC	98
		R-TTG TGG TGT ATG GGT TGA TTG GG

F = Forward primer (5′ to 3′); R = reverse primer (3′ to 5′).