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. Author manuscript; available in PMC: 2012 Oct 19.
Published in final edited form as: J Am Chem Soc. 2011 Sep 28;133(41):16428–16431. doi: 10.1021/ja208019p

Figure 2.

Figure 2

The dependence of the observed second-order rate constant (kcat/Km)obs for the proton transfer reactions of the free carbonyl form of [1-13C]-GA (20 mM total substrate) catalyzed by wildtype and L232A mutant Tbb TIM on the concentration of phosphite dianion in D2O at pD 7.0 (10 - 20 mM imidazole), 25 °C and I = 0.1 (NaCl). Key: (■) Data for wildtype Tbb TIM (Ref. 17); (●) Data for L232A mutant Tbb TIM.