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. Author manuscript; available in PMC: 2012 Oct 20.
Published in final edited form as: Neuron. 2011 Sep 21;72(2):245–256. doi: 10.1016/j.neuron.2011.09.011

Figure 5. Expanded GGGGCC hexanucleotide repeat forms nuclear RNA foci in human brain and spinal cord.

Figure 5

(A) Multiple RNA foci in the nucleus (stained with DAPI, blue) of a frontal cortex neuron of the proband of family 63 (63-1) using a Cy3-labeled (GGCCCC)4 oligonucleotide probe (red). (B) RNA foci observed in the nucleus of two lower motor neurons in FTD/ALS patient (13-7) carrying an expanded GGGGCC repeat using a Cy3-labeled (GGCCCC)4 oligonucleotide probe. (C) Absence of RNA foci in the nucleus of cortical neuron from FTLD-TDP patient (44-1) without an expanded GGGGCC repeat in C9ORF72. (D) spinal cord tissue sections from patient 13-7 that showed RNA foci with the (GGCCCC)4 oligonucleotide probe in (B) do not show any foci with a Cy3-labeled (CAGG)6 oligonucleotide probe (negative control probe). Scale bar: 10 μm (A and C), 20 μm (B and D).