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Isolation of ME1 and ME2 from the 200 mm cation-exchange fraction by reverse-phase column chromatography, as described in Methods. I, SDS-PAGE of the proteins separated after the reverse-phase HPLC step. Approximately 2 μg of protein was loaded per lane. II, Reverse-phase perfusion chromatogram. Approximately 300 μg of protein from the 200 mm NaCl fraction was injected into the column, and 100 μg of each fraction was collected.
