Table 2.
Envelope proteins of AngHV-1 as identified by 1D gel/nanoLC-MS/MS in the envelope fraction
| ORFa | NCBI ID | Descriptionb | Predicted molecular mass (kDa) | Number of peptidesc | Mascot score | CyHV-3d | Membrane protein type | Trans-membrane domain(s)e | Signal anchor or peptide | N-glycof | O-glycog |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 8 | 282174045 | 21.2 | 46 | 2390 | Type 1 | o1i | - | 4 | - | ||
| 49h | 282174086 | 26.4 | - | - | ORF83 | Type 3 | i4i | Signal peptide | - | - | |
| 51 | 282174088 | Major envelope protein | 26.5 | 137 | 5970 | ORF81 | Type 3 | i4i | Signal anchor | 1 | 1 |
| 66 | 282174103 | 42.7 | 1 | 46 | Type 1 | o1i | Signal peptide | 4 | 2 | ||
| 67 | 282174104 | WBV spike protein/Major glycoprotein | 152.9 | 54 | 2251 | ORF99 | Type 1 | o1i | Signal peptide | 13 | 8 |
| 71 | 282174108 | 11.4 | 29 | 1638 | Type 1 | o1i | Signal anchor | - | 2 | ||
| 78 | 282174115 | 16.9 | 8 | 609 | Type 3 | i2i | Signal peptide | - | - | ||
| 95 | 282174132 | ISAV HA | 41.6 | 190 | 11573 | Type 1 | o1i | Signal peptide | 5 | 1 | |
| 108 | 282174145 | ORF80 family | 108.2 | 4 | 183 | Type 1 | o1i | - | 3 | 56 | |
| 115 | 282174152 | 12.1 | 6 | 406 | Type 1 | o1i | Signal anchor | 2 | - | ||
| 125 | 282174162 | ORF109 family | 120.5 | 2 | 189 | Type 1 | o1i | Signal peptide | 16 | 16 |
athe ORF are ordered by number, bproperties newly suggested in this paper are presented in italics, cnumber of peptides and Mascot score in the envelope fraction, dhomologous ORF in CyHV-3 are given, in case of marginal sequence identity (E-value > 10-5 in a BLAST search limited to members of the Alloherpesviridae family) presented in italics, etransmembrane domain(s) and orientation: o = outside, i = inside, number refers to number of transmembrane domains, fpredicted N-glycosylation site(s), gpredicted O-glycosylation site(s), and hAngHV-1 ORF49 was only detected in very low abundance in complete virion preparations, and not in the envelope fraction.