Table 3.
Tegument proteins of AngHV-1 as identified by 1D gel/nanoLC-MS/MS in the capsid-tegument and envelope fraction
| ORFa | NCBI ID | Descriptionb | Predicted molecular mass (kDa) | Number of peptides (tegument)c | Mascot score | Number of peptides (envelope)d | Mascot score | CyHV-3e | Putative classificationf |
|---|---|---|---|---|---|---|---|---|---|
| 14 | 282174051 | ORF3 family | 32.3 | 2 | 63 | - | - | Tegument protein | |
| 16 | 282174053 | ORF13 family | 33.8 | 4 | 237 | 12 | 905 | Tegument-associated protein | |
| 17 | 282174054 | ORF13 family | 25.8 | 13 | 851 | 29 | 1496 | Tegument-associated protein | |
| 18 | 282174055 | 233.6 | 4 | 177 | - | - | ORF42 | Tegument protein | |
| 19 | 282174056 | 93.7 | 40 | 1717 | 7 | 372 | Tegument protein | ||
| 20 | 282174057 | 63.1 | 6 | 304 | - | - | ORF45 | Tegument protein | |
| 24 | 282174061 | ORF13 family | 29.3 | 12 | 842 | 39 | 2690 | Tegument-associated protein | |
| 26 | 282174063 | 18.4 | 1 | 38 | 2 | 74 | Tegument-associated protein | ||
| 30 | 282174067 | 118.4 | 94 | 5311 | 70 | 3350 | ORF97 | Tegument-associated protein | |
| 32 | 282174069 | 20.0 | 2 | 101 | 5 | 192 | Tegument-associated protein | ||
| 34 | 282174071 | 195.0 | 176 | 9205 | 167 | 8820 | ORF51 | Tegument-associated protein | |
| 35 | 282174072 | 33.4 | 12 | 576 | 46 | 2113 | ORF57 | Tegument-associated protein | |
| 38 | 282174075 | 44.1 | 10 | 455 | - | - | ORF70 | Tegument protein | |
| 39 | 282174076 | 62.7 | 1 | 65 | - | - | ORF69 | Tegument protein | |
| 40 | 282174077 | 162.7 | 101 | 4570 | 21 | 921 | Tegument protein | ||
| 43 | 282174080 | 18.0 | 18 | 1444 | 35 | 2491 | Tegument-associated protein | ||
| 81 | 282174118 | 51.5 | 10 | 561 | 48 | 2673 | ORF60 | Tegument-associated protein | |
| 83 | 282174120 | Cysteine protease domain/Large tegument protein | 376.8 | 538 | 28887 | 298 | 16343 | ORF62 | Tegument-associated protein |
| 103 | 282174140 | 25.0 | 5 | 273 | - | - | ORF91 | Tegument protein | |
| 114 | 282174151 | 20.7 | 19 | 913 | 61 | 3202 | Tegument-associated protein | ||
| 128 | 282174165 | 17.9 | 11 | 394 | 6 | 225 | Tegument-associated protein | ||
| 129 | 282174166 | 42.2 | 16 | 935 | 9 | 579 | Tegument-associated protein |
athe ORF are ordered by number, bproperties newly suggested in this paper are presented in italics, cnumber of peptides and Mascot score in the capsid-tegument fraction, dnumber of peptides and Mascot score in the envelope fraction, ehomologous ORF in CyHV-3 are given, in case of marginal sequence identity (E-value > 10-5 in a BLAST search limited to members of the Alloherpesviridae family) presented in italics, and fputative classification into a tegument or tegument-associated protein.