. 2011 Oct 5;42(1):105. doi: 10.1186/1297-9716-42-105

Table 4.

Structural proteins of AngHV-1 as identified by 1D gel and 2D nanoLC-MS/MS

ORF^a	NCBI ID	Location^b	Description^c	Predicted molecular mass (kDa)	1D gel/nanoLC-MS/MS^d			2D nanoLC-MS/MS^e
					Number of peptides	Mascot score	EmPAI	Number of peptides	Mascot score	EmPAI
8	282174045	Envelope	Membrane protein type 1	21.2	29	1138	1.3	37	1339	2.59
14	282174051	Tegument	ORF3 family	32.3	-	-	-	-	-	-
16	282174053	Tegument-associated	ORF13 family	33.8	12	349	0.88	11	465	0.66
17	282174054	Tegument-associated	ORF13 family	25.8	21	1010	0.51	10	459	0.70
18	282174055	Tegument		233.6	-	-	-	-	-	-
19	282174056	Tegument		93.7	5	179	0.17	13	713	0.30
20	282174057	Tegument		63.1	3	123	0.19	-	-	-
24	282174061	Tegument-associated	ORF13 family	29.3	11	493	1.07	11	471	0.59
26	282174063	Tegument-associated		18.4	-	-	-	-	-	-
30	282174067	Tegument-associated		118.4	23	801	0.54	16	450	0.34
32	282174069	Tegument-associated		20.0	5	239	1.41	-	-	-
34	282174071	Tegument-associated		195.0	71	2641	1.32	58	2200	0.71
35	282174072	Tegument-associated		33.4	19	797	1.35	18	632	0.67
36	282174073	Capsid	Capsid triplex protein 2	40.2	29	1016	3.99	22	856	1.80
38	282174075	Tegument		44.1	4	142	0.39	-	-	-
39	282174076	Tegument		62.7	2	57	0.12	-	-	-
40	282174077	Tegument		162.7	22	783	0.56	13	446	0.29
42	282174079	Capsid	Capsid triplex protein 1	42.9	20	979	1.73	17	558	1.42
43	282174080	Tegument-associated		18.0	4	121	0.48	8	291	1.12
48	282174085	Capsid		102.6	32	1112	1.18	26	876	0.97
49	282174086	Envelope^f	Membrane protein type 3	26.4	1	38	0.14	1	42	0.14
51	282174088	Envelope	Major envelope protein/Membrane protein type 3	26.5	30	1042	4.70	30	972	2.18
57	282174094	Capsid	Capsid protease-and-scaffolding protein	78.0	9	345	0.45	8	328	0.43
66	282174103	Envelope	Membrane protein type 1	42.7	6	458	0.18	-	-	-
67	282174104	Envelope	WBV spike protein/Major glycoprotein/Membrane protein type 1	152.9	25	948	0.43	15	577	0.26
71	282174108	Envelope	Membrane protein type 1	11.4	4	172	0.82	3	103	0.33
78	282174115	Envelope	Membrane protein type 3	16.9	4	146	0.23	-	-	-
81	282174118	Tegument-associated		51.5	7	226	0.63	1	56	0.07
83	282174120	Tegument-associated	Cysteine protease domain/Large tegument protein	376.8	166	6652	1.46	104	3870	0.71
95	282174132	Envelope	ISAV HA/Membrane protein type 1	41.6	54	2069	1.81	52	2256	1.48
100	282174137	Capsid		61.1	2	66	0.13	1	33	0.03
103	282174140	Tegument			-	-	-	-	-	-
104	282174141	Capsid	Major capsid protein	139.9	213	8402	5.33	104	4058	2.56
108	282174145	Envelope	ORF80 family	108.2	-	-	-	1	33	0.06
114	282174151	Tegument-associated		20.7	28	852	3.64	13	550	1.27
115	282174152	Envelope			-	-	-	-	-	-
125	282174162	Envelope	ORF109 family		-	-	-	-	-	-
126	282174163	Capsid		22.4	1	30	0.17	-	-	-
128	282174165	Tegument-associated		17.9	4	139	0.48	1	60	0.21
129	282174166	Tegument-associated		42.2	8	290	0.19	6	238	0.18

^athe ORF are ordered by number with the ORF only detected in the virion fractions presented in italics, ^blocation as determined in this paper, ^cproperties newly suggested in this paper are presented in italics, ^dnumber of peptides, Mascot score and emPAI in the complete virion fraction as determined by 1D gel/nanoLC-MS/MS, ^enumber of peptides, Mascot score and emPAI in the complete virion fraction as determined by 2D nanoLC-MS/MS, ^fAngHV-1 ORF49 was not detected in the envelope fraction and classified as an envelope protein only on the basis of predicted structural properties.