Figure 5.

Effect of Chx on mRNA expression patterns. A blot carrying 20 μg/lane of total aleurone RNA from de-embryonated half-seeds not treated (0; lanes 1, 5, and 9), treated with 50 μm Chx (C; lanes 2, 6, and 10), treated with 10⁻⁶ m GA (G; lanes 3, 7, and 11), or treated with GA plus Chx (GC; lanes 4, 8, and 12) for 6 h was probed sequentially with cDNAs for GAR-RNase, PAPI, and high-pI α-amylase (Amy). After the blot was washed at high stringency, images were captured and quantitated with a phosphor imager. The signals for PAPI in each lane were used to correct for differences in loading. RA, Relative abundance, where the value obtained with GA was set to 1.0.