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. 2011 Oct 27;6(10):e24923. doi: 10.1371/journal.pone.0024923

Figure 4. STAT3 activity down-regulates Necdin expression in human prostate cancer and breast cancer cell lines.

Figure 4

A. Western blot. Total protein from DU145 and PC3 cells treated with IL-6 (10 nmol/L) for 12 or 24 hours was analyzed by Western blot. B. Total protein was harvested from MDA-MB-468, MDA-MB-231 and MCF-7 cells and subjected to Western blot analysis. C. MCF-7 cells were seeded and allowed to adhere overnight before being transiently transfected with control (GFP), pMvSrc or pRc/CMV-STAT3-C plasmids using Lipofectamine PLUS. Total protein was collected at 48 h post-transfection and subjected to Western blot analysis. For densitometry, images were digitally scanned and optical density of the bands was quantified using Scion Image (Scion Corporation, Frederick, MD) and normalized to control.