Table 2. Csf1r-GFP Positive Cells Co-localize with Microglial markers.

Marker	Total cells counted	Total number of CSF-1R+ cells (green)	Total number of antibody labeled cells (red)	Total double labeled cells (yellow)	Percentage of co-localizing cells
MBP	120	54	66	0	0%
GFAP	131	127	4	0	0%
NeuN	504	27	477	0	0%
F480	68	68	68	68	100%
Iba-1	46	46	46	46	100%

Using Z stacks of immunostained brain sections on the confocal microscope as defined in the material and methods and from regions shown in Table S1, the coincidence of the immunostain with the GFP expressed from the Csf1r promoter was determined for the number of cells indicated in column 2.