Figure 6. Expression of CNGCs in cpr5.

Steady state levels of CNGC10, CNGC11, CNGC12, CNGC19, CNGC20 mRNA in leaves and roots of cpr5-2, cpr5-3 and wild-type Col-0 evaluated by qRT-PCR. Seedlings were germinated and grown on 1/20^th MS medium supplemented with 20 mM KCl. After two-week of growth shoot and root tissue was harvested and RNA extract for qRT-PCR. PP2A (At1g13320) was used as an endogenous reference gene for normalization across samples, and data presented as 2^−ΔCt. Error bars represent standard deviations calculated following [29].