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. 2011 Oct 27;6(10):e25637. doi: 10.1371/journal.pone.0025637

Figure 2. GP-BAR1 gene ablation predisposes to development of severe colitis in response to DSS in 3 months old mice C57Bl/6 mice.

Figure 2

Panel A–C. GP-BAR1−/− mice develop a severe disease (n = 6; P<0.05), while changes of intestinal permeability were increased in GP-BAR1−/− mice in comparison to wild type mice (n = 10; *P<0.05 versus naive; **P<0.05 versus wild type DSS). Panel D. Immuno-histochemical detection of GP-BAR1 expression in the colon of wild type mice challenged with DSS. GP-BAR1 is abundantly expressed in mononuclear cells infiltrating the lamina propria. Original magnification 20× and 40× (inset). Panel E. Colon expression of GP-BAR1 mRNA in wild type mice challenged with DSS (n = 6; P<0.05). Panel F. Treatment of GP-BAR1−/− mice with DSS results in a dramatic and diffuse loss of alcian blue-positive mucous cells. Original magnification 10×.