Figure 2. Presynaptic reduction of dTip60 in the nervous system leads to an expansion of synaptic boutons at larval NMJs.

Flies homozygous for either dTip60^E431Q, dTip60^RNAi or control w¹¹¹⁸ were crossed to flies homozygous for the nervous system elav^C155 pan-neuronal GAL4 driver, and staged third instar progeny larvae were collected. Confocal imaging analysis of larval boutons on muscles 6/7 at abdominal segment A4 immunohistochemically stained with anti-HRP (green) that labels the entire presynaptic membrane. (A) control w¹¹¹⁸ larvae (B) larvae expressing dTip60^RNAi (C) larvae expressing dTip60^E431Q. Scale bar 10 um. Line arrow depicts Ib bouton, thick arrow depicts Is bouton. Histogram depicts quantitative analysis of bouton number on muscles 6 and 7 at abdominal segment 4 where (D) represents total bouton number (E) number of Is boutons (F) number of Ib boutons and (G) percentage of satellite boutons. Small arrow depicts type Is bouton, line arrow depicts type Ib bouton. In the analyses, w¹¹¹⁸ genotype is represented by 18 larval preparations (n = 18), dTip60^RNAi (n = 19) and dTip60^E431Q (n = 19). Asterisks (*) indicates statistically significant difference in relation to control where single asterisks indicate p≤0.001 and double asterisks indicate p≤0.0001. All error bars depict standard error of the mean.