Figure 6. The number of presynatpic futsch-positive loops are increased at synapses in mutant dTip60 larvae.

Confocal imaging analysis of larval boutons on muscles 6/7 at abdominal segment 4 immunohistochemical double labeled with HRP antibody (green) that labels the entire presynaptic membrane and Futsch antibody (red) that labels Futsch stained microtubule loops. (A,B) is w¹¹¹⁸ (C,D) is dTip60^E431Q (E,F) is dTip60^RNAi. Histogram depicts quantitative analysis of Futsch-positive loop number on muscles 6 and 7 at abdominal segment A4 where (G) is total number of loops. In the analyses, w¹¹¹⁸ genotype is represented by 30 larval preparations (n = 30), dTip60^RNAi (n = 25) and dTip60^E431Q (n = 27). Small thick arrows depict Futsch stained microtubule loops (C), line arrows (E) depict Futsch stained microtubule rearrangement within loops. Scale bar is 10 um. Asterisks (\) indicates statistically significant difference in relation to control where double asterisks indicate p≤0.001 and three asterisks indicate p≤0.0001. All error bars depict standard error of the mean.