Table 1. Results of MS BLAST searches using de novo peptide sequences against the ButterflyBase Database and BLASTP searches using ButterflyBase protein sequences against UniRef100.
spot | ButterflyBase identificationa | Length predicted polypeptide (aa)b | MS BLAST Scorec | Peptidesd | BLASTp vs Uniref100 (top hit)e | |||
Description | species | Accession | E-value | |||||
2 | STP00012_1 | 636 | 206 | 8 | Ecdysone oxidase | Spodoptera littoralis | Q95NZ0 | 0 |
5 | ni | - | - | - | - | - | - | - |
6 | BMP000597_1 | 331 | 280 | 7 | oxidase/peroxidase | Nasonia vitripennis | UPI00015B588D | 4,00E-48 |
7 | PXP00618_1 | 188 | 207 | 4 | Antennal esterase CXE3 | S. littoralis | D3GDL4 | 5,00E-82 |
8 | PXP00109_1 | 629 | 821 | 11 | Heat shock cognate 70 | Plutella xylostella | Q2WG65 | 0 |
15 | ni | - | - | - | - | - | - | - |
16 | ni | - | - | - | - | - | - | - |
20 | SFP04769_1 | 244 | 362 | 7 | Juvenile hormone binding protein | Heliothis virescens | Q25175 | 2,00E-88 |
22 | AYP00014_1 | 164 | 193 | 3 | Elongation factor 1 beta (Fragment) | Antheraea yamamai | Q6L609 | 1,00E-43 |
23 | HMP00286_1 | 243 | 308 | 4 | Proteasome subunit alpha type | Bombyx mori | Q2F661 | e-128 |
24 | ni | - | - | - | - | - | - | - |
25 | ni | - | - | - | - | - | - | - |
26 | SFP00311_3 | 175 | 389 | 4 | Translationally-controlled tumor protein homolog | B.mori | Q75VN3 | 4,00E-91 |
27 | MSP00420_1 | 193 | 63 | 1 | Brasiliensin | Triatoma brasiliensis | A7YIH7 | 2,00E-32 |
28 | SEP00040_1 | 160 | 115 | 2 | Eukaryotic translation initiation factor 5A | Spodoptera exigua | P62924 | 1,00E-89 |
29 | ni | - | - | - | - | - | - | - |
31 | TBP00299_1 | 111 | 160 | 2 | Group 15 allergen protein | Dermatophagoides pteronyssinus | Q4JK70 | 2,00E-07 |
34 | SFP11092_1 | 301 | 462 | 9 | Hemolymph proteinase | Manduca sexta | Q5MPB6 | 8,00E-61 |
40 | SFP04769_1 | 244 | 273 | 5 | Juvenile hormone binding protein | H. virescens | Q25175 | 2,00E-88 |
43 | LOP00661_1 | 177 | 328 | 5 | Putative uncharacterized protein | Branchiostoma floridae | C3YWF0 | 7,00E-56 |
44 | SFP01905_2 | 255 | 114 | 2 | Phosphoglycerate mutase | B. mori | Q3S2I9 | e-132 |
45 | BMP020939_1 | 262 | 317 | 6 | PREDICTED: similar to AGAP001957-PA | Tribolium castaneum | UPI0000D56B2F | e-122 |
47 | ni | - | - | - | - | - | - | - |
49 | PIP01950_1 | 161 | 251 | 4 | Actin-depolymerizing factor 1 | B. mori | Q1HPP5 | 8,00E-79 |
50 | HMP01674_3 | 151 | 403 | 6 | Actin-depolymerizing factor 1 | B. mori | Q1HPP5 | 1,00E-79 |
51 | HEP01070_2 | 218 | 158 | 3 | - | - | - | - |
55 | PIP04233_1 | 189 | 78 | 1 | GF16309 | Drosophila ananassae | B3LX08 | 8,00E-67 |
60 | HAP00799_1 | 297 | 511 | 9 | Glyceraldehyde-3-phosphate dehydrogenase | B. mori | Q1EPM0 | e-152 |
61 | HAP00799_1 | 297 | 461 | 6 | Glyceraldehyde-3-phosphate dehydrogenase | B. mori | Q1EPM0 | e-152 |
62 | BMP006112_1 | 176 | 375 | 5 | GTP-binding nuclear protein | B. mori | Q2F5M1 | 2,00E-97 |
64 | TNP00255_1 | 167 | 287 | 4 | Peptidyl-prolyl cis-trans isomerase | B. mori | Q5CCL3 | 2,00E-79 |
65 | LOP00067_1 | 165 | 150 | 3 | Peptidyl-prolyl cis-trans isomerase | B. mori | Q5CCL3 | 2,00E-79 |
Cluster ID of best hit in ButterflyBase by MS BLAST (ni = not identified).
Number of amino acids of predicted ButterflyBase protein.
MS BLAST scoring.
Number of peptides matching best hit in ButterflyBase in MS BLAST search.
Results of blastp search using ButterflyBase predicted protein against UniRef100.