Table 1. Results of MS BLAST searches using de novo peptide sequences against the ButterflyBase Database and BLASTP searches using ButterflyBase protein sequences against UniRef100.

spot	ButterflyBase identificationa	Length predicted polypeptide (aa)b	MS BLAST Scorec	Peptidesd	BLASTp vs Uniref100 (top hit)e
					Description	species	Accession	E-value
2	STP00012_1	636	206	8	Ecdysone oxidase	Spodoptera littoralis	Q95NZ0	0
5	ni	-	-	-	-	-	-	-
6	BMP000597_1	331	280	7	oxidase/peroxidase	Nasonia vitripennis	UPI00015B588D	4,00E-48
7	PXP00618_1	188	207	4	Antennal esterase CXE3	S. littoralis	D3GDL4	5,00E-82
8	PXP00109_1	629	821	11	Heat shock cognate 70	Plutella xylostella	Q2WG65	0
15	ni	-	-	-	-	-	-	-
16	ni	-	-	-	-	-	-	-
20	SFP04769_1	244	362	7	Juvenile hormone binding protein	Heliothis virescens	Q25175	2,00E-88
22	AYP00014_1	164	193	3	Elongation factor 1 beta (Fragment)	Antheraea yamamai	Q6L609	1,00E-43
23	HMP00286_1	243	308	4	Proteasome subunit alpha type	Bombyx mori	Q2F661	e-128
24	ni	-	-	-	-	-	-	-
25	ni	-	-	-	-	-	-	-
26	SFP00311_3	175	389	4	Translationally-controlled tumor protein homolog	B.mori	Q75VN3	4,00E-91
27	MSP00420_1	193	63	1	Brasiliensin	Triatoma brasiliensis	A7YIH7	2,00E-32
28	SEP00040_1	160	115	2	Eukaryotic translation initiation factor 5A	Spodoptera exigua	P62924	1,00E-89
29	ni	-	-	-	-	-	-	-
31	TBP00299_1	111	160	2	Group 15 allergen protein	Dermatophagoides pteronyssinus	Q4JK70	2,00E-07
34	SFP11092_1	301	462	9	Hemolymph proteinase	Manduca sexta	Q5MPB6	8,00E-61
40	SFP04769_1	244	273	5	Juvenile hormone binding protein	H. virescens	Q25175	2,00E-88
43	LOP00661_1	177	328	5	Putative uncharacterized protein	Branchiostoma floridae	C3YWF0	7,00E-56
44	SFP01905_2	255	114	2	Phosphoglycerate mutase	B. mori	Q3S2I9	e-132
45	BMP020939_1	262	317	6	PREDICTED: similar to AGAP001957-PA	Tribolium castaneum	UPI0000D56B2F	e-122
47	ni	-	-	-	-	-	-	-
49	PIP01950_1	161	251	4	Actin-depolymerizing factor 1	B. mori	Q1HPP5	8,00E-79
50	HMP01674_3	151	403	6	Actin-depolymerizing factor 1	B. mori	Q1HPP5	1,00E-79
51	HEP01070_2	218	158	3	-	-	-	-
55	PIP04233_1	189	78	1	GF16309	Drosophila ananassae	B3LX08	8,00E-67
60	HAP00799_1	297	511	9	Glyceraldehyde-3-phosphate dehydrogenase	B. mori	Q1EPM0	e-152
61	HAP00799_1	297	461	6	Glyceraldehyde-3-phosphate dehydrogenase	B. mori	Q1EPM0	e-152
62	BMP006112_1	176	375	5	GTP-binding nuclear protein	B. mori	Q2F5M1	2,00E-97
64	TNP00255_1	167	287	4	Peptidyl-prolyl cis-trans isomerase	B. mori	Q5CCL3	2,00E-79
65	LOP00067_1	165	150	3	Peptidyl-prolyl cis-trans isomerase	B. mori	Q5CCL3	2,00E-79

^aCluster ID of best hit in ButterflyBase by MS BLAST (ni = not identified).

^bNumber of amino acids of predicted ButterflyBase protein.

^cMS BLAST scoring.

^dNumber of peptides matching best hit in ButterflyBase in MS BLAST search.

^eResults of blastp search using ButterflyBase predicted protein against UniRef100.