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. 2011 Oct 27;6(10):e26660. doi: 10.1371/journal.pone.0026660

Figure 5. Overview of the affinity pull-down assay using PR-linked resin.

Figure 5

Paromomycin was bound to AminoLink Plus coupling resin. The PR-linked resin (experimental) and unlinked resin (used as negative control) were incubated with AG83 whole cell lysate. The beads were then washed and the resin was loaded onto 10% SDS-PAGE gel. The gel was stained with sypro ruby and the fragments were excised and digested with trypsin. The protein samples were analyzed by LC-MS/MS and the results compared to determine the proteins that interacted with PR selectively.