Table 2. Proteins downregulated in the paromomycin resistant (PRr) strain.
| S. No. | Accession No. | Name of the Protein(Down-regulated) | PRr:WT ratio | P-value | Percent coverage | Total Number of Peptides |
| Translation | ||||||
| 1 | LinJ11_V3.1160 | Eukaryotic release factor 3, putative | 0.17 | 1.45E-03 | 5.3 | 3 |
| 2 | LinJ18_V3.0630LinJ36_V3.3950 | 60S ribosomal protein L10a, putative | 0.24±0.2 | 1.99E-01 | 22 | 5 |
| 3 | LinJ22_V3.1370LinJ22_V3.1410 | 40S ribosomal protein L14, putative | 0.23±0.1 | 8.20E-03 | 5.2 | 4 |
| 4 | LinJ30_V3.0470 | Aspartyl-tRNA synthetase, putative | 0.09±0.01 | 8.88E-06 | 3.6 | 3 |
| 5 | LinJ35_V3.2040 | 60S ribosomal protein L32 | 0.27±0.2 | 1.88E-01 | 31.6 | 5 |
| Cell cytoskeleton | ||||||
| 6 | LinJ14_V3.1190 | Kinesin K39, putative | 0.3±0.04 | 2.40E-02 | 7.3 | 2 |
| Metabolic Enzymes | ||||||
| Glycolysis | ||||||
| 7 | LinJ20_V3.0110LinJ20_V3.0120 | Phosphoglycerate kinase C, glycosomal | 0.1±0.1 | 6.67E-02 | 19.1 | 14 |
| Krebs cycle | ||||||
| 8 | LinJ35_V3.0850 | NADH-dependent fumarate reductase-like protein | 0.47±0.07 | 1.62E-01 | 2.3 | 3 |
| 9 | LinJ35_V3.1190 | NADH-dependent fumarate reductase, putative | 0.24±0.02 | 5.57E-03 | 8.5 | 6 |
| Electron Transport Chain | ||||||
| 10 | LinJ05_V3.0500LinJ05_V3.0510 | ATPase alpha subunit | 0.45±0.27 | 3.12E-01 | 18.8 | 16 |
| 11 | LinJ12_V3.0620 | Cytochrome c oxidase subunit iv | 0.25±0.28 | 2.61E-01 | 7.4 | 2 |
| Other Metabolic Enzymes | ||||||
| 12 | LinJ31_V3.2320 | 3,2-trans-enoyl-CoA isomerase, mitochondrial precursor, putative | 0.39±0.03 | 6.69E-02 | 8.8 | 2 |
| 13 | LinJ35_V3.3390 | 6-phosphogluconate dehydrogenase, decarboxylating putative | 0.36±0.03 | 4.79E-02 | 8.6 | 3 |
| Chaperones/Stress Proteins | ||||||
| 14 | LinJ21_V3.1330 | t-complex protein 1, delta subunit, putative | 0.47±0.03 | 1.40E-01 | 12 | 4 |
| 15 | LinJ35_V3.3900 | t-complex protein 1, eta subunit, putative | 0.42±0.1 | 1.09E-01 | 15.6 | 6 |
| DNA/RNA Processing | ||||||
| 16 | LinJ15_V3.1500 | Proliferative cell nuclear antigen (PCNA), putative | 0.27±0.1 | 2.87E-02 | 11.6 | 3 |
| 17 | LinJ19_V3.0090 | Fibrillarin, putative | 0.45±0.1 | 1.69E-01 | 12.2 | 2 |
| 18 | LinJ32_V3.0410 | ATP-dependent RNA helicase, putative | 0.27±0.2 | 1.58E-01 | 11.9 | 6 |
| Proteins Involved in Intracellular Survival/Processing | ||||||
| 19 | LinJ35_V3.0070 | Prohibitin, putative | 0.4±0.03 | 7.79E-02 | 7.9 | 2 |
| Hypothetical Proteins | ||||||
| 20 | LinJ10_V3.0910 | Hypothetical protein, conserved | 0.47±0.1 | 1.48E-01 | 12.8 | 2 |
| 21 | LinJ35_V3.0140 | Hypothetical protein, conserved | 0.22±0.2 | 1.61E-01 | 21.8 | 3 |
List of proteins with decreased expression levels in the PR resistant strain.
Fold change of protein level measured by SILAC with standard deviation is given as PRr:WT ratio.
p-value reported here were analyzed and validated by PeptideProphet.