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. 2011 Oct 27;6(10):e27020. doi: 10.1371/journal.pone.0027020

Figure 6. Calpastatin overexpression in the synovial fibroblasts of RA patients efficiently suppressed IL-6 production.

Figure 6

(A) Synovial fibroblasts derived from RA patients were subjected to mock- or CS-dIV-GRV infection, and the infected cells were sorted by FACS. Sorting purity was >99%. (B) The sorted GFP+ cells in panel A were seeded into 96-well plates at 1000 cells/well. Forty-eight and 72 hours after seeding, ATP was measured using a CellTiter-Glo® Assay kit. Summarized data from three independent experiments are shown. (C) The cells used in panel B were stimulated with the indicated concentration of LPS for 24 hours, and IL-6 in the culture supernatant was measured by ELISA. Similar results were obtained in three independent experiments.