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. 2011 Oct 27;6(10):e26998. doi: 10.1371/journal.pone.0026998

Figure 2. Analysis of specific mRNA transcript expression in cultured trout and chicken erythrocytes after PAMP or cytokine stimulation.

Figure 2

(a) Response of trout and (b) chicken erythrocytes after 12 hours exposure to: 50 µg/ml of LPS, 50 µg/ml of poly (I∶C), 5 µg/ml of PGN and 50 ng/ml of rTNF. CCL4, Mx, IFNα, TLR3 and TLR9 (TLR21 in chicken) mRNA abundance was analyzed by RT-PCR and 1.2% agarose gel electrophoresis. One representative of 3 and 4 individuals is shown for trout and chicken respectively. (c) RT-PCR analysis of the tEC response over time (6–24 h) to 50 µg/ml of poly (I∶C), densitometry data shown as fold changes (mean ± std.dev., n = 4 individuals) with respect to 18S rRNA. (d) Absolute Q-PCR analysis of the tEC response over time control (6–24 h) to 50 µg/ml of LPS, fold changes (mean ± std.dev., n = 4 individuals) in respect to specific transcript copy number.