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. 2011 Oct 27;6(10):e26998. doi: 10.1371/journal.pone.0026998

Figure 3. Analysis of polysome-bound mRNA regulation in cultured trout erythrocytes after poly (I∶C) stimulation.

Figure 3

(a) RT-PCR analysis of polysome-bound mRNAs (AIF-1, TNFR-like, IRF1.1 and IFNα) under control and after poly (I∶C) stimulation. M, ribosome free mRNA; D, mono and disome bound mRNA; and P, polysome bound mRNA obtained after polysome gradient centrifugation. NSC119889 (200 µM) was added to inhibit polysome formation. (b) Densitometry analysis (n = 3) of % bound or non-polysome specific mRNA in respect to the total specific mRNA abundance.