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. 2011 Oct 27;7(10):e1002339. doi: 10.1371/journal.ppat.1002339

Figure 5. SOX depletes polyribosomes and cosediments with 40S subunits.

Figure 5

(A) TREx BCBL-1-RTA (BCBL-1) cells were mock treated (latent) or induced (lytic) with 1 µg/ml doxycycline, 500 ng/ml ionomycin, and 20 ng/ml 2-O-tetradecanoylphorbol-13-acetate for 24 h to stimulate KSHV replication, then subjected to sucrose gradient fractionation using a 15–60% sucrose gradient in order to monitor the abundance of translating polysomes. (B) Fractions collected from the induced BCBL-1 gradients shown in panel A were Western blotted with the indicated antibodies. (C) Lysate from induced BCBL-1 cells was fractionated using a 5–20% sucrose gradient and analyzed by Western blot with indicated antibodies. (D) 293T cells were transfected with the indicated construct and subjected to sucrose gradient fractionation to obtain polysome profiles. Dashed lines indicate polysome levels of either latent cells (A) or vector expressing cells (D).