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. Author manuscript; available in PMC: 2011 Oct 28.
Published in final edited form as: J Invest Dermatol. 2010 Oct 7;131(3):753–761. doi: 10.1038/jid.2010.300

Figure 1. Purified pdg proteins retain activity in vitro after addition of NLS and TAT peptides to enhance cellular delivery.

Figure 1

a. Human keratinocytes and skin fibroblasts were transfected with expression vectors for GFP, pdg-GFP, or pdg-NLS-GFP and GFP localization was visualized by direct immunofluoresence microscopy. DAPI (blue) indicates nuclei. Scale bar = 10 μm. b. Coomassie blue stained 15% SDS-PAGE gel showing the purity of pdg, pdg-NLS, or pdg-NLS-TAT enzymes after protein expression and purification. c. Plasmid nicking assay on UVC-irradiated DNA showing in vitro activity of the purified pdg enzymes after addition of NLS and TAT peptides. I = covalently closed circular, supercoiled plasmid DNA; II = nicked duplex DNA; III = linear duplex DNA; (-) = plasmid not treated with a pdg.