Figure 4.

Complementation of the tps1Δtps2Δ mutant by SlTPS1 under the control of the CUP1 promoter. Yeast mutants lacking the TPS1 protein (tps1Δ) or both TPS1 and TPS2 (tps1Δtps2Δ) were transformed with pSTS1 (pSAL4 containing SlTPS1), pSTS2 (pSAL4 containing ScTPS1), or pSAL4 vector alone and spread on 2% agar plates in minimal medium without uracil (−ura) supplemented with 2% Gal (SGal), 2% Glc (SGlc), or 2% Glc with copper sulfate (SGlc +100 μm CuSO₄). The wild-type control strain W303-1A (WT) was also transformed with pSAL4.