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Complementation of the tps1Δ mutant by SlTPS1 under the control of the PMA1 promoter. The yeast mutant lacking TPS1 protein (tps1Δ) was transformed with pRTS1 (pRS6 containing SlTPS1), pRTS2 (pRS6 containing ScTPS1), or pRS6 vector alone and spread on 2% agar plates in minimal medium without His (−his) supplemented with 2% Gal (SGal) or 2% Glc (SGlc). The wild-type control strain W303-1A (WT) was also transformed with pRS6.
