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SlTPS1 restores the osmotolerance defect of a yeast tps1Δ deletion mutant. Osmotolerance assay of a tps1Δ deletion mutant transformed with the SlTPS1 gene. Yeast mutants lacking the protein TPS1 (tps1Δ) were transformed with pRTS1 (containing SlTPS1), pRTS2 (containing ScTPS1), or pRS6 vector alone and spotted on 2% agar plates in rich YPGal medium supplemented with 0.9 m NaCl or 1.6 m sorbitol. The wild-type control strain W303-1A (WT) was also transformed with pRS6.
