Table I.
Trehalose content of the transformed tps1Δ and tps1Δtps2Δ mutants with pSAL4-derived vectors
| Carbon Source | Strain | Trehalose | Trehalose Content |
|---|---|---|---|
| μmol g−1 yeast wet wt | % | ||
| Gal | Wild type (pSAL4) | 71.70 ± 25.18 | 100 |
| tps1Δtps2Δ (pSAL4) | 5.25 ± 6.77 | 7 | |
| tps1Δtps2Δ (SlTPS1) | 6.32 ± 6.23 | 9 | |
| tps1Δtps2Δ (SlTPS1ΔC) | 2.13 ± 0.52 | 3 | |
| tps1Δtps2Δ (ScTPS1) | 19.27 ± 14.93 | 27 | |
| tps1Δ (pSAL4) | 4.80 ± 4.90 | 7 | |
| tps1Δ (S1TPS1) | 2.83 ± 1.65 | 4 | |
| tps1Δ (ScTPS1) | 48.43 ± 21.16 | 67 | |
| Glc | Wild type (pSAL4) | 50.90 ± 9.02 | 100 |
| tps1Δtps2Δ (SlTPS1) | 5.42 ± 4.58 | 11 | |
| tps1Δtps2Δ (ScTPS1) | 18.50 ± 12.93 | 36 | |
| tps1Δ (ScTPS1) | 36.48 ± 10.69 | 72 | |
| Fru | Wild type (pSAL4) | 81.77 ± 31.03 | 100 |
| tps1Δtps2Δ (SlTPS1) | 6.95 ± 5.01 | 7 | |
| tps1Δtps2Δ (ScTPS1) | 21.13 ± 13.04 | 26 | |
| tps1Δ (ScTPS1) | 54.40 ± 24.92 | 66 |
Three independent transformants of wild-type yeast, the tps1Δ mutant, or the tps1Δtps2Δ mutant transformed with pSAL4 vector alone or harboring the SlTPS1, SlTPS1ΔC, or ScTPS1 genes were grown in minimal medium with the indicated carbon source plus 100 μm CuSO4. Trehalose content was determined in the stationary phase (7.0 A600). Values are means ± sd.