Table II.
Trehalose content of the transformed tps1Δ mutant with pRS6-derived vectors
| Carbon Source | Strain | Trehalosea | Trehalose Content |
|---|---|---|---|
| μmol g−1 yeast wet wt | % | ||
| Gal | Wild type (pRS6) | 16.45 ± 4.59 | 100 |
| tps1Δ (pRS6) | 1.45 ± 0.07 | 9 | |
| tps1Δ (SlTPS1) | 16.55 ± 0.35 | 100 | |
| tps1Δ (SlTPS1ΔC) | 6.59 ± 0.72 | 40 | |
| tps1Δ (ScTPS1) | 26.20 ± 2.97 | 159 | |
| Glc | Wild type (pRS6) | 29.45 ± 0.64 | 100 |
| tps1Δ (SlTPS1) | 18.95 ± 5.87 | 64 | |
| tps1Δ (ScTPS1) | 27.85 ± 7.42 | 95 | |
| Fru | Wild type (pRS6) | 29.00 ± 7.07 | 100 |
| tps1Δ (SlTPS1) | 20.40 ± 3.39 | 70 | |
| tps1Δ (ScTPS1) | 34.05 ± 4.59 | 117 |
Three independent transformants of wild-type yeast or the tps1Δ mutant transformed with pRS6 vector alone or harboring the SlTPS1, SlTPS1ΔC, or ScTPS1 genes were grown in minimal medium with the indicated carbon source.
a
Trehalose content was determined in the stationary phase (7.0 A600). Values are means ± sd.