Table III.
Viability of tps1Δ mutant cells transformed with SlTPS1 gene after thermoinduction
| Strain | Surviving Cells |
|---|---|
| % | |
| Wild type (pRS6) | 127.00 ± 19.52 |
| tps1Δ (pRS6) | 29.67 ± 6.66 |
| tps1Δ (SlTPS1) | 80.00 ± 21.16 |
| tps1Δ (ScTPS1) | 96.00 ± 2.65 |
Thermoinduction was performed by incubating yeast cells in liquid SGal (-His) medium up to approximately 0.4 A600 at 25°C, and then shifted for 1 h at 39°C. Aliquots for colony counting were taken just before and after thermoinduction. Data represent the mean of three independent transformants and their sd.