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. Author manuscript; available in PMC: 2012 Aug 9.
Published in final edited form as: Oncogene. 2011 Jul 18;31(6):716–727. doi: 10.1038/onc.2011.273

Figure 8. Vav3 and AR are recruited to the same transcriptional complexes in the PSA enhancer.

Figure 8

A. LNCaP-R1 cells with endogenous Vav3 expression were maintained in hormone-depleted medium for 72h and then were treated with vehicle or 1nM R1881 for 16h. Chromatin immunoprecipitation (ChIP) was conducted using anti-AR (rabbit), anti-Vav3 (rabbit) antibody, or corresponding rabbit IgG control. Vav3 recruitment to the PSA distal enhancer was determined by real-time PCR. Data plotted as “% input” are the average of two independent experiments. B. LNCaP/Vav3-Flag cells were subjected to sequential ChIP (re-ChIP) assay with anti-AR antibody or IgG control followed by anti-Flag antibody. Vav3 co-recruitment with AR to the PSA distal enhancer was determined by real-time PCR. Data plotted as “Fold ± SEM” relative to vehicle control is the average of three independent experiments. Significance was determined using a two-tailed Student’s t test (*p<0.05).