Figure 4.

Stability of soybean root GS holoenzyme in the native and the oxidized form incubated in root extract. The 4-d-old root extract was incubated in a DTT-mediated MCO system in the presence (DTT/Fe-EDTA) or absence of EDTA (DTT/FeCl₃), and samples were removed at the indicated times. The reaction was terminated by the addition of glycerol to 20%, followed by immediate freezing. A, Results of native gel electrophoresis (at 4°C) followed by western blotting and immunodetection using anti-GS antibodies. 8er., Octamer; 4er., tetramer. B, Bands quantified using Intelligent Quantifier software and plotted. The figure represents typical results.