Figure 1.

Clathrin-Hub1 Expression Inhibits Endocytosis but Not Vacuolar Transport in Arabidopsis Protoplasts.

(A) and (B) Staining of the PM directly after FM4-64 addition (A) and endocytic uptake of the dye after 30 min incubation (B).

(C) Protoplasts expressing the GFP-tagged clathrin-Hub1 (GFP-Hub1) were stained with FM4-64 and incubated for 30 min. The dye is not internalized and remains at the PM.

(D) Comparative quantification of intrinsic FM4-64 signals from 20 protoplasts either in the presence or absence of GFP-Hub1. Error bars indicate the sd of signal numbers.

(E) Coexpression of the soluble vacuolar marker spRFP-AFVY and cytosolic GFP (cytGFP) in protoplasts. spRFP-AFVY is efficiently transported into the lumen of the vacuole, even if fluorescent cytosolic proteins (cytGFP) are coexpressed.

(F) Coexpression of GFP-Hub1 and the soluble vacuolar marker spRFP-AFVY in protoplasts. The presence of GFP-Hub1 in the cytosol does not perturb vacuolar transport of spRFP-AFVY (cf. to [E]).

Bars = 5 μm.