Figure 5.

NATA1 Expression and N^δ-Acetylornithine Affect Arabidopsis–M. persicae Interactions.

(A) Expression of NATA1 in dissected leaf blades and midveins (inset), with and without 24 h MeJA elicitation. Mean ± se of n = 4 or 5.

(B) N^δ-acetylornithine in petiole exudates and aphids feeding for 1 d from plants treated 4 d earlier with MeJA. Mean ± se of n = 3.

(C) NATA1 expression with 25 aphids feeding on one leaf. Fold induction, with 0 h set to 1. Mean ± se of n = 3 or 4.

(D) N^δ-acetylornithine accumulation in Col-0 wild type after 4 d of M. persicae feeding. Mean ± se of n = 3.

(E) Aphid reproduction on artificial diet containing 0 (control), 0.5, 5, or 10 mM N^δ-acetylornithine. Mean ± se of n = 8.

(F) M. persicae reproduction on detached nata1-1 leaves with petioles in a tube containing N^δ-acetylornithine (see inset). Number of aphid progeny after 4 d. Mean ± se of n = 22.

(G) M. persicae reproduction wild type and nata1-1 after MeJA treatment. Mean ± se of n = 19.

(H) M. persicae reproduction on N. tabacum transiently expressing p35S:NATA1. Number of aphid progeny after 7 d. Mean ± se of n = 25.

(I) M. persicae reproduction on Arabidopsis wild-type Col-0 and nata1 mutant transformed with p35S:NATA1. Number of aphid progeny after 7 d. Mean ± se of n = 25 to 27. ND, not detected. *P < 0.05, t test relative to control samples.