Table 2.
Complementation Analysis of the pod1/POD1 Mutant
| T2 Transgenic Plants | Kanr | Kans | Kanr/Kans |
| Genomic POD1-6 | 234 | 149 | 2.3:1 |
| Genomic POD1-10 | 204 | 109 | 1.87:1 |
| Genomic POD1-18 | 130 | 55 | 2.36:1 |
| Genomic POD1-20 | 314 | 98 | 3.2:1 |
| Genomic POD1-21 | 434 | 241 | 1.8:1 |
| Genomic POD1-26 | 220 | 101 | 2.2:1 |
| pLAT52:POD1 | 1324 | 676 | 1.96:1 |
| pPOD1:RXRRR#x003ERXAAA | 1186 | 600 | 1.97:1 |
| pPOD1:#x0394LR | 841 | 450 | 1.86:1 |
Constructs as indicated were transformed into pod1-1/POD1-1 background, and kanamycin selection was performed in T2 progeny. Genomic POD1 construct contains 1.628-kb promoter, coding region (including exons and introns), and 482-bp sequence downstream stop codon. The suffix numbers 6, 10, 18, 20, 21, and 26 indicate different transgenic lines. pLAT52:POD1 refers to POD1 CDS driven by LAT52 promoter. pPOD1:POD1RXRRR>RXAAA and pPOD1:POD1ΔLR refer to mutated POD1 CDS driven by the POD1 promoter, respectively. The Kanr/Kans ratio was obtained from at least five independent transgenic plants.