Table 2.
Genetic Complementation of the msh1 Phenotype with Plastid MSH1-GFP Gene Constructs
| Hemicomplementation with Chloroplast-Targeting MSH1 Gene Construct | ||||||||
| Construct | Pop | MM | Mm | mm/va | Pχ2(1:2:1) | Ta+/vb | T−/v | Pχ2(3:1) |
| RuBP-MSH1-GFP | T1 | 61 | 97 | 42/5 | 0.15 | |||
| MSH1-GFP T1-104(mm grn) | T2 | 87 | 60/7 | 27/23 | 0.19 | |||
| MSH1-GFP T1-117(mm var) | T2 | 78 | 62/2 | 16/14 | 0.36 | |||
Transgenes were introduced to MSH1/msh1 heterozygous plants, with normal (χ2 1:2:1, 3:1) Mendelian segregation (MM, Mm, mm) of the resulting populations. T2 populations display cosegregation for the transgene (T) and complemented phenotype.
Number of plants showing evidence of variegation.
T designates transgene.