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. 2011 Sep 6;23(9):3215–3229. doi: 10.1105/tpc.111.088492

Figure 3.

Figure 3.

The Temperature Effect on the Expression of MFT.

(A) MFT expression levels in mature embryos. Wheat cultivars SK and N61 grown at 13 and 25°C after anthesis. qRT-PCR was performed. Mature seeds grown at 13°C were isolated at DAA80 in SK and at DAA76 in N61. Mature seeds grown at 25°C were isolated at DAA43 in SK and at DAA39 in N61. The relative mRNA levels at 25°C were set at a standard value of 1. Transcript levels were normalized using the actin gene as an internal control. Results from quintuple independent biological samples (n = 5) are shown, and error bars represent sd.

(B) Time course of MFT expression levels during seed development in SK and N61. qRT-PCR was performed with triplicate technical samples (n = 3), and equivalent results were obtained using duplicate independent biological samples. The error bars represent sd. Transcript levels were normalized using the actin gene as an internal control. In SK, the relative mRNA level at 25°C at DAA43 was set at a standard value of 1. In N61, the relative mRNA level at 25°C at DAA39 was set at a standard value of 1. Closed circles, grown at 13°C; open squares, grown at 25°C.