Figure 1.
JAZ Domain Structure and JA-Ile–Triggered Degradation by the Ubiquitin-Proteasome Pathway.
(A) Schematic representation of the JAZ1 protein and its conserved domains. Known protein interactors and functions are depicted. The TIFY motif for the ZIM domain and the JAZ degron for the Jas domain are underlined. Important amino acids mentioned in the text are in bold.
(B) In the absence of JA-Ile, JAZ binds MYC2 and represses gene expression. COI1-SKP may form a substrate adaptor that, with JA-Ile and InsP5 as a cofactor, binds the Jas domain of JAZ proteins. Upon formation of the SCFCOI1 E3 ligase complex, JAZ1 is presumably polyubiquitinated, which marks it for degradation by the 26S proteasome.
aa, amino acid; NT, N-terminal domain.