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. 2011 Sep 9;23(9):3547–3559. doi: 10.1105/tpc.111.088351

Figure 5.

Figure 5.

Time Course of B-Dependent NIP5;1 mRNA Degradation in Col-0 Roots and Effect of +182 to +200 bp Substitution in the 5′ UTR.

(A) A schematic representation of growth conditions. Col-0 plants were grown with 0.3 μM B for 21 d on solid medium and then transferred to hydroponic culture containing 0.3 μM B for 2 d. Transgenic plants were grown with 0.3 μM B for 14 d on solid medium. Plants were transferred to hydroponic medium containing 0.3 or 100 μM B for 10 min and then cordycepin was applied in the hydroponic culture. Root samples for Col-0 and whole-plant samples for transgenic plants were harvested at 0, 10, and 30 min, and 20 min, respectively, after cordycepin treatment, and mRNA levels were measured by qRT-PCR.

(B) Col-0.

(C) and (D) Transgenic plants carrying P35SUTR+7-GUS shown as +7 (C) and P35SUTR+7Δ+182 to +200-GUS shown as +7Δ+182 to +200 (D). The RNA levels at each time point were normalized to the amount at the zero time point and plotted on a log scale to show mRNA decay. Means of three biological replicates ± sd (n = 3) are shown.