Subcellular Localization of NHX1 and NHX2.
(A) NHX2-associated YFP fluorescence in mature root cells of stably transformed plants expressing 35S-NHX2-YFP. Arrows point to nuclei.
(B) Transient expression of Ub10-NHX1-GFP in Arabidopsis cotyledons.
(C) to (E) Transient coexpression of Ub10-NHX1-GFP and 35S-NHX2-RFP in Arabidopsis cotyledons.
(F) to (H) Transient expression of Ub10-NHX1-GFP in the Ub10-VAMP711-RFP background.
(C) and (F) are GFP images; (D) and (G) are RFP images; (E) and (H) are merged images. Arrowheads in (B), (E), and (H) point to the tonoplast and transvacuolar cytoplasmic strands.
Bar in (A) = 50 mm; bars in (B) to (H) = 20 mm.