Figure 3.

Immunohistochemical localization of PGRMC1 in bovine ovarian cortex. PGRMC1 is localized in primordial, primary and secondary follicles (A), in early antral follicles (B) and in antral follicles (C). Note that the expression of PGRMC1 is more evident in the granulosa cells closer to the oocyte, in the GV and in the internal theca layer. PGRMC1 was detected in the endothelial cells (arrows) and in the germinal epithelium at the ovarian surface (D, arrowhead). No immunoreactivity was seen in negative control where incubation with the primary antibody had been omitted (E). Final magnification, 200×; scale bar, 50 µm.