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. 2011 May 4;8(65):1748–1759. doi: 10.1098/rsif.2011.0114

Figure 1.

Figure 1.

In vitro and in vivo characterization of PNiPAAm. Schematic of thermo-responsive behaviour of PNiPAAm with CII (a). Below LCST (approx. 32°C) (b), 100 µg of PNiPAAm mixed with 100 µg of CII (1 mg ml−1) remains in solution and above this temperature forms a lattice incorporating CII; in vitro release profile of CII from PNiPAAm at 37°C (c); percentage of released CII retaining native CII conformation from PNiPAAm–CII mixture (100 µg each at 1 : 1 ratio) in vitro (d); serum antibody response recognizing native and/or denatured conformation of CII (units per ml) (e). Optimization of PNiPAAm concentration (50–200 µg) for immunization mixed with 100 µg of CII (f) after 21 days of injection. Error bars denote ±s.e.m. (e) Black bars, native CII; white bars, denatured CII.