Supplementary Figure 2
Tail-tip fibroblasts (TTFs) from 14-month-old mdx mice show high proliferation potential, and give rise to stable iPSCs
(A) 14m-TTFs and MEFs exhibit similar proliferation ability. MEFs, 14m-TTFs, and 14m-MuFs were seeded at 2.5x104 cells /well on 12-well plates. Cell numbers were counted every day. Bar indicates mean + s.d. (n=3/group).(B) 14m-TTFs and MEFs show similar reprogramming efficiency. Four days after infection of a cocktail of retrovirus vectors (OKSM+DsRed), DsRed-positive cells were re-seeded at 1.35x104 cells/6-cm dish on feeder cells. Ten days later, colonies were stained for alkaline phosphatase (AP). Reprogramming efficiencies are shown as AP+ colonies per seeded cells. Bar indicates mean + s.d. (n=3/group). **p<0.01 (t-test).
(C) Representative AP and Nanog staining of 14m-TTF-iPSCs.
