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. Author manuscript; available in PMC: 2012 Oct 26.
Published in final edited form as: J Am Chem Soc. 2011 Oct 4;133(42):16809–16811. doi: 10.1021/ja2081873

Table 1.

Oxidative Half-reaction rate constants.

Enzyme kox (M−1s−1) kox (wild-type)/kox (mutant)
Wild-type FAOXa 1.6 × 105 b
Lys276Met FAOXa 291 550
Lys53Met FAOXa 5.6 × 103 b 29
Wild-type L. lactis DHOD 3.0 × 103
Lys43Met L. lactis DHOD 2.6 × 103 1.2
Wild-type E. coli DHOD 6.2 × 104 c
Wild-type E. coli DHOD•Orotate 5.0 × 103 c 12.4 d
E. coli Lys66Met 6.2 × 104 1.0
E. coli His19Asn 3.7 × 104 1.7
E. coli Arg102Met 5.9 × 104 1.1
a

FAOX, fructosamine oxidase.

b

Ref 23.

c

Ref 25.

d

This is kox (free wild-type)/kox (orotate complex).