Fig. 12.
STC-1 conditioned media (cm) inhibits LPS-stimulated IL-12 p40 and nitric oxide (NO) production in macrophages. A–E: 4-day conditioned media from STC-1 cells were added to RAW264.7 cells (A), murine peritoneal macrophages (B and C), or murine bone marrow (BM)-derived macrophages (D and E). A: 4-day conditioned media from the IEC line Caco-2 and epithelial cell line HEK-293T were used as controls. Macrophages were subsequently stimulated with 1 μg/ml LPS for 24 h (A, B, and D) or 48 h (C and E). Experiments were performed in trip-licate, and results represent means ± SD. **P < 0.01 compared with media control for each group.