The murine EEC line STC-1 expresses TLRs. A, top: expressions of TLR and MD2 mRNA were analyzed by PCR of reverse-transcribed total RNA isolated from STC-1 cells. RNA isolated from RAW264.7 macrophage cells was used as a positive control. RT-PCR amplified β-actin mRNA is shown as a loading control (bottom). B–E: STC-1 cells were stained (FITC secondary) with control IgG (B), anti-TLR2 (C), anti-TLR4/MD2 complex (D), and anti-TLR6 antibodies (E). Experiments were repeated 3 times, and a representative result is displayed.