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. Author manuscript; available in PMC: 2011 Oct 28.
Published in final edited form as: Am J Physiol Gastrointest Liver Physiol. 2007 Mar 29;292(6):G1770–G1783. doi: 10.1152/ajpgi.00249.2006

Fig. 5.

Fig. 5

The murine EEC line STC-1 expresses TLRs. A, top: expressions of TLR and MD2 mRNA were analyzed by PCR of reverse-transcribed total RNA isolated from STC-1 cells. RNA isolated from RAW264.7 macrophage cells was used as a positive control. RT-PCR amplified β-actin mRNA is shown as a loading control (bottom). B–E: STC-1 cells were stained (FITC secondary) with control IgG (B), anti-TLR2 (C), anti-TLR4/MD2 complex (D), and anti-TLR6 antibodies (E). Experiments were repeated 3 times, and a representative result is displayed.