Fig. 8.

TLR ligands induce TNF and macrophage inhibitory protein (MIP)-2 mRNA and protein expression in STC-1 cells. STC-1 cells were stimulated with 10 μg/ml of LPS or sBLP for 0–4 h. A: cells were harvested via TRIzol. Total RNA was isolated, digested with DNase I, and subsequently reverse transcribed into cDNA. TNF, MIP-2, and β-actin PCR primer sequences are described in MATERIALS AND METHODS. Experiments were repeated 3 times, and a representative result is displayed. B–E: STC-1 cells were stimulated with increasing amounts of LPS (B and D) or sBLP (C and E) for 48 h. ELISA was performed on cell-free supernatants for MIP-2 (B and C) and TNF (D and E). Experiments were performed in triplicate, and results represent means ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with unstimulated cells for each group.