. Author manuscript; available in PMC: 2011 Oct 28.

Published in final edited form as: Am J Physiol Gastrointest Liver Physiol. 2007 Mar 29;292(6):G1770–G1783. doi: 10.1152/ajpgi.00249.2006

Table 1.

Primers used for RT-PCR on murine genes

	Primers		Annealing Temperature, °C	Cycle Number	Product Size, bp
Gene	Forward	Reverse	Annealing Temperature, °C	Cycle Number	Product Size, bp
TLR1	5′-CCGAATCTCTTCGGCACGTT-3′	5′-TAACTTGGGACGGGCACAGC-3′	58	38	501
TLR2	5′-ATGGTGACCTCCGAGCGTGT-3′	5′-GAGCCTCGGAATGCCAGCTT-3′	60	36	571
TLR4	5′-GGCATGGCATGGCTTACACC-3′	5′-ATTCTCCCAAGATCAACCGATGG-3′	58	38	500
TLR6	5′-TGCTCCCAGTTGCTCACTTGC-3′	5′-GGGCACACCATGTGGATGAA-3′	60	36	559
MD2	5′-TTCTGCAACTCCTCCGATGCAA-3′	5′-TCCCTCGAAAGAGAATGGTATTGATG-3′	58	36	300
TNF	5′-CCAGGCGGTGCCTATGTCTC-3′	5′-AGCAAATCGGCTGACGGTGT-3′	60	32	407
MIP-2	5′-GTGCTGCACTGGTCCTGCTG-3′	5′-GGCTTCAGGGTCAAGGCAAA-3′	58	36	221
TGF-β	5′-CTCCCACTCCCGTGGCTTCT-3′	5′-GCTCTGCACGGGACAGCAAT-3′	58	38	427
β-Actin	5′-CCTAAGGCCAACCGTGAAAAG-3′	5′-TCTTCATGGTGCTAGGAGCCA-3′	56	21	646

TLR, Toll-like receptor; MIP, macrophage inhibitory protein; TGF, transforming growth factor.