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. Author manuscript; available in PMC: 2012 Sep 20.
Published in final edited form as: Biochemistry. 2011 Aug 26;50(37):7881–7890. doi: 10.1021/bi200837b

Table 1.

Results of global NLLS analysis of 9 independent RSCt-DNA binding isotherms measured in the presence of varying concentrations of ADP in the range from 0.1 mM to 1.0 mM using equations (2) through (6). Column A corresponds to global fits in which the variable A (see materials and methods) was allowed to float and column B corresponds to global fits in which the variable A was fixed to unity.

A B
K1 (M−1) (10.6 ± 0.6) × 106 (9.0 ± 0.6) × 106
K2 (M−1) (5.0 ± 1.2) × 104 (7 ± 2) × 104
K3 (M−1) (9 ± 3) × 103 (2.2 ± 0.6) × 104
K4 (M−1) (2.01 ± 0.13) × 106 (2.7 ± 0.1) × 106
A 1.28 ± 0.05 1.0 (fixed)
s 0.160 ± 0.013 0.177 ± 0.013
Variance 8.5 × 10−6 1.2 × 10−5