Figure 3.

Single turnover ligation kinetics of the Schist26 hammerhead ribozyme. A) A 20% denaturing polyacrylamide gel used to separate ³²P-labeled ligated full-length substrate from ³²P-P1 in the ligation experiment for Schist26 in 10 mM Mg²⁺. The different lanes correspond to samples removed from the ligation reaction at various times after addition of Mg²⁺. B) Plot of the fraction of ligated substrate vs. time where the solid line shows the fit to a single exponential with a k_{obs, ligate} of ~26 min⁻¹.